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candida from the departments of Nario and Putumayo in Colombia

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Last year, a survey showed that Americans believe that cannabis is less dangerous than alcohol or tobacco

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The lysate was cleared by ultracentrifugation and batch-bound to HisPur Cobalt Resin (Thermo, 89964) equilibrated with binding buffer (20 mM Tris-HCl pH 7.2, 500 mM NaCl, 10 mM imidazole and 5% glycerol, pH adjusted to 8.0) for 30 min in the cold room with rocking