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To obtain airway epithelium by brushing, disposable brushes (2.0 mm, Ballard Medical, Draper, UT) were advanced through the working channel of the bronchoscope and used to brush and collect airway epithelial cells from 2 nd or 3 rd order bronchi from the right lower lobe by gently gliding the brush back and forth 10 times as previously described [3], [5], [41]

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