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Cell cycle analysis Following treatment with DMSO or disulfiram (25, 50, or 100 nM) for 48 h, IGR-N91 cells were fixed and stained with bromodeoxyuridine (BrdU) and/or 7-amino-actinomycin (7-AAD, BD Pharmingen BrdU Flow Kit)

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doi: 10.1016/j.bmcl.2019.07.051 72 JiangZPflugKUsamaSMKuaiDYanXSitcheranRet al

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The safety profile is comparable across injection, oral, and sublingual forms

One contributing factor may be differences in cell-type composition, as IPF cultures contained fewer ciliated and more secretory cells (Fig