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An antibacterial agent cocktail [22] (AC, a mixture of 1 mg ampicillin per mL water, 1 mg metronidazole per mL water and 1 mg neomycin per mL water) was added to the drinking water for 14 days, which was followed by 0.5 mg vancomycin per mL water for another 14 days [18, 19, 21, 22]

As you can expect from above, changes will be gradual and incremental

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AQP4 knockout aggravates ischemia/reperfusion injury in mice

The rise in KYN level in KYN level is at least in part contributed by the rise in IDO1 and TDO2 in livers and linked to hepatic inflammation