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Finally, immunofluorescence analysis of the cells was performed using confocal microscopy (Axio-lmager-LSM-800 Germany)

It has been reported that the excessive ROS generation and metal ions released from internalized metal-based NPs have an impact on multiple cell signaling pathways, mainly including Janus kinase/signal transducers and activators of transcription (JAK-STAT), nuclear factor-kappa-lightchain enhancer of activated B cells (NF-B), Phosphatidylinositol 3-kinase (PI3K)/protein serine threonidase (Akt), mitogen-activated protein kinase (MAPK), and nuclear factor erythroid 2-related factor 2 (Nrf2) (Ko et al., 2016

Glutathione, one of the most abundant cellular metabolites, maintains cellular environment in reduced state, protecting proteins and iron-sulfur clusters from oxidative stress and is essential for biosynthesis reactions, such as nucleotide synthesis 3

Gluoxidant-A is a health supplement that marks the first of its kind in India