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From 1998 to 2004, the amount of nicotine in cigarettes increased by an average of 10 percent, according to a 2006 study by the Massachusetts Department of Public Health, though its not clear why or even if such an increase makes cigarettes more addictive

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Commonly, this manifests as fine lines around the eyes (crows feet) and mouth (smokers lines) at an earlier age than non-smokers

Interestingly, authors showed that, despite significant differences in nicotine concentration, cytotoxic profile was consistent between the two systems

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Using the universal primers ITS1ITS4, the pathogens genomic DNA was amplified through PCR