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Results In vivo proteolysis of substrates with different recognition sequences by the pTEV + protease First, we wanted to know to which extent different amino acids at the P1' position of the recognition sequence influence proteolytic activity of the pTEV + protease, which has higher processivity due to removal of the last 8 amino acids and increased substrate affinity by the p14-SF3B155 381-424 domains

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