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Methotrexate is administered intramuscularly at a dose of 50 mg/m 2 of body surface area

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Ethical approval Ethical approval was not required as the study did not involve human participant

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Leaf tissues encompassing zones agroinfiltrated with these constructs were collected at 5 DPI, total protein extracted and the epitope tagged proteins affinity purified by using either agarose resins possessing epitope tag-specific antibody or Strep-Tactin matrix columns