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A 25 L reaction mixture was prepared, comprising 12.5 L PCR Master Mix (2X) (Thermo Scientific), 0.8 L of 10 mM forward primer, 0.8 L of 10 mM reverse primer, 2 L MT-DNA (20 ng/L), and 8.9 L nuclease-free water

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In recent days, agency officials have convened hourslong meetings grappling with how to implement the six-page memo, which breaks with longstanding FDA policy requiring scientific verification of health benefits for smokers before any new products are introduced

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