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Prediction analyses were performed through backward stepwise Cox proportional hazards regression and expressed as the hazard ratio (HR)

Routine Integration: Morning: Cleanser Toner Eye Cream Moisturizer SPF Evening: Cleanser Toner Serums (optional) Moisturizer Eye Cream Focus Area: Apply to under-eye area, orbital bone, and inner corners where dark circles concentrate

doi: 10.1016/j.clnesp.2024.07.017 236

The supernatants were collected by centrifugation at 5000 rpm for 5 min at 4 C and precleared by incubated with GFP-Trap agarose beads (Chromotek) or HA-Beads (Roche) for 2 h at 4 C with rotation