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Various factors contribute to skin darkening, including hormonal changes, lifestyle habits like smoking, exposure to the sun, genetics, and environmental pollutants like pollution and unhealthy diets

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Since its discovery in ox bile in 1827, several physiological functions have been described for the amino acid, ranging from the classical role of conjugating agent for bile acids, to wider actions as osmotic pressure regulator, modulator of calcium homeostasis and signaling and, more recently, as an endogenous anti-oxidant and anti-inflammatory compound in various tissues