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Chromatographic separation was carried out using an UltiMate 3000 HPLC system (Thermo Fisher Scientific, Rockford, IL, USA) fitted with a YMC-Triart PFP column (3 mm particle size, 12 nm pore size, 150 2.1 mm

Adult motor neuron apoptosis is mediated by nitric oxide and Fas death receptor linked by DNA damage and p53 activation

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The 3D structure of the GPx3 mutant with all Cys converted to Ser was refined by MD simulations and analyzed with Profile-3D and Procheck following the method as described previously 31,32

This method increased the sulfadiazine water solubility by 50 times, resulting in increased sulfadiazine release and antibacterial action