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CHIR99021 was removed after 72 h and DE cells were cultured in RPMI/B27 + Insulin for 24 h ( For growth factor Definitive Endoderm (DE) differentiation, human iPSCs cultured in 6-well dishes to 60% confluence were washed sequentially with DPBS / and DMEM/FI2 medium (Thermo Fisher Scientific)

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We've troubleshooted countless issues for researchers, and a pattern of common, avoidable mistakes has emerged