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2005) and in the plasma (Monserrat-Mesquida et al

You can really run into a lot of trouble and to, I dont want to say harm, but really not be of much help

Subsequently, cells were centrifuged at 4 C, 4,000 g for 15 min, then resuspended in cold buffer A (20 mM HEPES-Na + , pH 8.0, 500 mM NaCl, 1 mM TCEP) and sonicated three times for 15 min each