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Conclusion: MTHFR gene mutation testing can provide valuable insights, but its clinical utility and necessity remain debated

Toxicity of intermediates was higher than that of the parent molecule (assessment with the use of Microtox bioassay)

Malic enzymes ME participate in reactions that link the components of catabolic metabolism in glycolysis and the Krebs cycle via the oxidative decarboxylation of malate to pyruvate, thereby inducing the anabolic metabolism with concomitant NADPH production