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The mechanisms underlying these effects include the inhibition of protein tyrosine phosphatase 1B (PTP1B), which is physiologically responsible for insulin receptor inhibition through dephosphorylation and attenuates insulin signal transduction (123, 124)

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After thawing, cells were resuspended in 83 mmol/L sucrose, 10 mmol/L HEPES, pH 7.2 and homogenized by 30 strokes using pestle B in a 2 mL Dounce homogenizer