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After washing, cells were stained with Alexa Fluor 488conjugated anti-rabbit (1:200, Invitrogen, A-10055) and Alexa Fluor 594conjugated anti-mouse (1:200, Invitrogen, A-21207) secondary antibodies for 1 h at RT

The research has shown that anatabine, an alkaloid present in Solanaceae plants, improves the mouse model of Hashimotos thyroiditis (129)

Inhibiting the release and uptake of KRASG12D can suppress macrophage-mediated tumor growth in pancreatic cancer [109]

Oxidative stress parameters were systematically assessed by measuring plasma catalase (CAT) activity, superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), total antioxidant capacity (T-AOC), and the lipid peroxidation marker malondialdehyde (MDA) using standardized assay kits (Nanjing Jiancheng Bioengineering Institute, China)

371,372 They are crucial in tumorigenesis, invasion, metastasis, and chemoresistance, and may serve as novel diagnostic biomarkers and anticancer targets

This may involve the use of naturally occurring antioxidants or completely synthetic molecules