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But the brain fog, the pins and needles in my hands and the fact that I needed coffee just to feel normal it didnt feel right. Hed tried vitamins from the pharmacy

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For immunofluorescence staining, cells were rinsed three times with PBS before being incubated with BODIPY TM 581/591 C11 dye in serum-free medium at 37 C with 5% CO2 for 30 min before being visualized under a fluorescence microscope (Evos FL, Thermo Fischer Scientific) with objective 40