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In this study, disruption of SIRT1 expression revealed that the impact of GADD45 on hepatic lipid synthesis was mediated through the SIRT1/AMPK signaling pathway

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Retrieved June 10, 2025, from Drugs..com

Following this, cells were washed with permeabilization buffer (Biolegend) and then stained with intracellular antibody cocktail ( Table 1 ) for 30 mins on ice, wash with Biolegend permeabilization buffer then stained with metal-conjugated streptavidin for 10 mins on ice

Trends in Endocrinology & Metabolism Sampson et al