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All models included sex (male serving as reference) and age as fixed effect predictors

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Thus, K2:TEV was cloned into pCRII-TOPO (Invitrogen) by TOPO cloning between the XhoI and SpeI

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Biomed Pharmacother 111:568578 Silva FH, Claudino MA, Calmasini FB, Alexandre EC, Franco-Penteado C, Burnett AL, Antunes E, Costa FF (2016) Sympathetic hyperactivity, increased tyrosine hydroxylase and exaggerated corpus cavernosum relaxations associated with oxidative stress plays a major role in the penis dysfunction in Townes sickle cell mouse