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Characterization of macrophage infiltration and polarization by double fluorescence immunostaining in mouse colonic mucosa

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Normally, mucins are gradually released into the airways, where they absorb water and form a thin layer of protective mucus that traps pathogens and is easily cleared by cilia

The absorbance at 490 nm was measured using a multi-mode microplate reader

Feasibility depends on clear responsibilities, adequate resources, and meaningful engagement with all affected groups