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3T3-L1 pre-adipocytes cells were cultured with standard culture media (DMEM, 4.5 g/L glucose, L-glutamine, sodium pyruvate, 10% FBS, 1% antibiotic-antimycotic solution) and grown for 48 h before initiating differentiation using the manufacturer's suggested protocol and modified from previous published work

I came in the first time completely run down and left already feeling better

Switching up where you inject helps avoid soreness and ensures the shot works well

What they did: Obese mice received AOD-9604 orally (via gavage) for 14 days at various doses (50, 250, 500 g/kg)

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