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& Bonini-Domingos, C

Lane 1: Molecular weight marker (#09547-74) Lane 2: Serum Lane 3: 0.1 M Gly pH 2.8 Lane 4: 0.1 M Gly pH 3.4 Lane 5: 0.1 M Gly pH 4.0 Lane 6: Arg-Antibody Elution Buffer Note that lanes 3, 4, 5 and 6 are independent results of the above procedure using different elution buffers

In addition to RSL3, other synthetic small molecules, including DPI7, DPI, JKE1674, and JKE-1716, can inhibit GPX4 activation (26)

While AMD affects central vision, most people retain some level of peripheral vision

GPX1-4 and GPX6 use selenocysteine as the active center to catalyze the reduction of H 2 O 2 or organic hydroperoxides to water or corresponding alcohols, thereby reducing their toxicity and maintaining redox balance

The non-specific ROS probe 2,7-Dichlorodihydrofluorescein diacetate (DCFH-DA) was prepared as a 100 mM stock in DMSO and stored as frozen aliquots