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RNA-seq Analysis Liver tissue from young (5 months) and old (20 months) wt and C/EBP uORF mice (from six individuals per group) was homogenized on ice with a motor driven pellet pestle (Kontes) in the presence of QIAzol reagent (Qiagen) and total RNA was isolated as described in Zidek et al(Zidek et al., 2015)