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Recent studies have extensively investigated ferroptosis/cuproptosis-related compounds or components, which exhibit notable anti-cancer effects in HNSCC, as summarized in Table 2

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Brain tissue section preparation and immunofluorescence After the animals had been sacrificed as described above, the brain tissues were removed, postfixed with 4% PFA overnight, and cryoprotected in 30% (w/v) sucrose in PBS 35

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the label names what to use and in what volume, commonly sterile water for injection, and that specification is part of the product rather than a detail left to the bench