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This enabled us to remove the metabolic effect from the use of different media

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Cells were plated on 48-well plates, coated with anti-CD3 (1 g ml 1 ) and anti-CD28 (10 g ml ) antibodies, in RPMI medium or DMEM, supplemented with 10% FCS, penicillin, streptomycin, HEPES, glutamine, pyruvate, nonessential amino acids and -mercaptoethanol diluted with PBS (1:1), in the presence of IL-2 (10 ng ml 1 , Peprotech) and IL-12 (10 ng ml 1 , Peprotech) (T H 1 conditions), with or without amino acids

Key Functional Ingredients Include: Folate : Provided as L-5-methyltetrahydrofolate calcium salt for active folate support

1a), we prioritized EMC3, which enabled the purification of ER with markedly reduced contamination from other organelles (Extended Data Fig

36 ), suggesting a less significant effect of the nuclear location and deacetylase activity