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After incubation, the coverslip was transferred into the original medium for 15 min and for labeling, neurons were three times washed with 1x PBS and fixed with 4 % PFA/ 4 % sucrose in PBS for 15 min at room temperature (RT)

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Storage conditions for some special products should refer to their storage details.

Calcium levels were corrected for albumin using the following equation: Ca corr = total calcium (0.025 albumin) + 1

When Edema Worsens: Monitoring Fluid Status on Semaglutide While most patients see edema improvement, some experience temporary fluid retention early in semaglutide therapy due to slower initial weight loss or increased oral food intake during titration