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Probe labeling For intrinsic cysteine reactivity, protein lysates were separated into two identical aliquots and incubated with either 10 M or 100 M IPM at room temperature (RT, ~25 C) for 1 h with rotation and light protection

Grny M, Bilska-Wilkosz A, Iciek M, Hereta M, Kamiska K, Kamiska A, Chwatko G, Rog Z, Lorenc-Koci E: Alterations in the Antioxidant Enzyme Activities in the Neurodevelopmental Rat Model of Schizophrenia Induced by Glutathione Deficiency during Early Postnatal Life

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Extends life to 28 days

10.1038/srep15809 40 KirtikarK