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Gut microbiota profiling by 16S rRNA gene sequencing To profile the microbial composition, faecal samples were subjected to total genome DNA extraction using the QIAamp DNA Stool Mini Kit following the manufacturers instruction (Qiagen, Venlo, The Netherlands)

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The presence of PML bodies characterises ALT activity in cells (Chung et al 2012)

The main phase 2 publication described cagrilintide as well tolerated overall and reports adverse events within the trial context