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30,42 Experimental Evidence from Medicinal Plants in Diabetic Wound Healing In vivo studies using streptozotocin (STZ)-induced diabetic rat and mouse models have demonstrated improved wound contraction, epithelialisation, and collagen deposition following treatment with medicinal plant extracts

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Nonspecific bound proteins were washed out with buffer A supplemented with 25 mM imidazole (Thermo Scientific, A10221) and laccases were eluted with buffer A containing 250 mM imidazole

However, the apparent increase in NP hydrodynamic diameter is several times than that of the original NP size, meaning that other mechanisms can contribute to this increase (NP agglomeration due to their colloidal destabilization, molecular bridge formation by the PC, itself)