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It is important to note, since Glx/tNAA indexes the cortical glutamate + glutamine pool rather than synaptic glutamate release alone, the attenuation we observed may reflect metabolic changes as well as altered neurotransmitter cycling, consistent with C-MRS evidence that prefrontal tricarboxylic (TCA)-cycle activity can fall without a corresponding change in glutamate and glutamine cycling in depression 36

Importantly, the dose of ferrous iron sulfate used in these studies was 0.15 mmol/kg FeSO 4 (human equivalent dose: 1.85 mg/kg), which is less than the recommended dose for an iron supplement (1 tablet with 325 mg FeSO 4 = 5.4 mg/kg)

They are strictly intended for laboratory research and in vitro experimentation