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The following day, the sections were washed in Duolink Wash Buffer A (0.01 M Tris-Base, 0.15 M NaCl, 0.05% Tween-20, pH 7.4), followed by incubation in Duolink PLA MINUS (Sigma Aldrich, DUO82004) and PLUS probes (Sigma Aldrich, DUO82002) at 37 C for 1 h

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Biochemistry 29 , 1000910018

In the absence of sufficient vitamin B12, the body is incapable of converting homocysteine, which consequently results in elevated levels of this amino acid

R., Meuleman, C., Demeyere, S., Lesaffre, E., & Cornillie, F

Participants who escalated too quickly experienced significantly higher rates of treatment discontinuation due to intolerable side effects