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Electrochemical oxidation induced selective tyrosine bioconjugation for the modification of biomolecules

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A SequestHT search against the SwissProt human database was performed and search parameters included a precursor tolerance of 10 ppm and a product ion tolerance of 0.6 Da

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After appropriate treatments, cells were washed in PBS and then lysed in 100 to 150 l of buffer (100 mM NaPO 4 , 1 mM ethylenediaminetetraacetic acid (EDTA), pH 7.5) containing 0.1% Triton X-100 and frozen at -80C until analysis