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Pretreatment mitochondrial priming correlates with clinical response to cytotoxic chemotherapy

Long-Term Medication Users Medications such as metformin (used for diabetes) and proton pump inhibitors (used for acid reflux) can interfere with B12 absorption when taken long term, making supplementation necessary

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Research applications Research applications In vitro cellular migration and scratch assays Endothelial angiogenesis and tube-formation studies Cell migration and cytoskeletal dynamics assays Biochemical response and epithelial integrity signaling models Combination mechanism-of-action and pathway interaction analyses For laboratory research use only

In rats, GCLC has a Km for glutamate that is about 10-fold higher than that of the GCL holoenzyme, which is higher than the cellular glutamate concentration in most tissues GCL is specific for the glutamyl moiety and is regulated physiologically by: (a) non-allosteric feedback competitive inhibition by glutathione (GSH) (Ki = 2.3 mM) which involves binding of GSH to the glutamate and another site on the enzyme [8, 9] and (b) availability of its precursor, cysteine [1]