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Cell viability assay Cell proliferation was detected via a CCK-8 assay (Biosharp, China) or a CellTiter-Glo assay (Promega) according to the manufacturers instructions

The benzyl alcohol preservative inhibits bacterial growth during this period but does not provide indefinite protection

The cost of the pen device itself, exclusive of the drug it contains, is estimated at $5-15 per unit

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But if its close to your next scheduled dose, skip the missed dose and take only the regular dose